| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Clostridium tepidum | GCTTCTGTTTGCCCTGTGG | GTAACTCCACCACCGGACA | 59.34 | 58.95 | 215 | 137 |
100.00%
|
100.00%
|
| Primer 12 | Clostridium tepidum | ACCAGAGTGGGTTAAAACACC | GCGCTTCCTTTGTTGAGCAG | 58.06 | 60.39 | 260 | 137 |
100.00%
|
100.00%
|
| Primer 13 | Clostridium tepidum | ACCCCAGTATGAACCGCAAA | TGTTGCTCAGTTCCTTTTGCA | 59.60 | 58.90 | 200 | 137 |
100.00%
|
100.00%
|
| Primer 14 | Clostridium tepidum | AACCCCAGTATGAACCGCAAA | TGTTGCTCAGTTCCTTTTGCA | 60.20 | 58.90 | 201 | 137 |
100.00%
|
100.00%
|
| Primer 15 | Clostridium tepidum | AGACGTGCAGGACAGTTGG | TTGCAAAAGAGGTTCCCCAC | 59.93 | 58.59 | 192 | 136 |
100.00%
|
100.00%
|
| Primer 16 | Clostridium tepidum | AGGAAGACGTGCAGGACAG | TTGCAAAAGAGGTTCCCCAC | 59.33 | 58.59 | 196 | 136 |
100.00%
|
100.00%
|
| Primer 17 | Clostridium tepidum | GCTTCTGTTTGCCCTGTGG | ACCTGCTTGCTTACATGCCA | 59.34 | 60.25 | 288 | 135 |
100.00%
|
100.00%
|
| Primer 18 | Clostridium tepidum | AGGCACTAGACATAGAGCTGC | TGCCCATTCTACCACCTGC | 59.31 | 59.70 | 214 | 135 |
100.00%
|
100.00%
|
| Primer 19 | Clostridium tepidum | GGAAGTAGAAGAGGCTCCAGG | ACGCTAGCGGTCCAATAGG | 59.24 | 59.26 | 155 | 133 |
100.00%
|
100.00%
|
| Primer 20 | Clostridium tepidum | TGTACTTGGTAGCGGAGATGA | TCAAGGCAAGTTCCACAGC | 58.27 | 58.28 | 207 | 132 |
100.00%
|
100.00%
|